The Wallabies have been dealt a further blow with coach Eddie Jones confirming Will Skelton and Taniela Tupou will miss next Monday’s (AEST) match with Wales.
Skelton was given until the last moment before he was officially ruled out, having injured at calf at training.
The Wallabies skipper was spotted walking around in a moon boot as he watched from the sidelines in their defeat to Fiji.
Tupou meanwhile was ruled out during the week after tweaking his hamstring.
With the clash against Wales crucial for their World Cup aspirations, Jones confirmed they will remain on the sidelines whilst not ruling them out for the rest of the tournament.
"Neither of those will be available for Wales,” he said.
“We can't blame the loss on Skelton not being there. We've got to be good enough to be able to cope with that.”
In a boost, Jones confirmed vice-captain Tate McDermott is progressing through the concussion protocol and will be fit to play.
Pone Fa'amausili will also be looking to make his return after missing the opening two games.
The loss to Fiji has the Wallabies’ World Cup hanging in the balance, needing a win against a Welsh side coming off wins against the Fijians and Portugal.
"I think they’ve gone back to a traditional style of Welsh play,” he said.
“A lot of the play comes off (Dan) Biggar. Their defence has improved and their lineout has improved. They’re big changes in their game since Warren has taken charge.
“They’ll be a tough team to beat."
Wales are coming off a hard-fought win over Portugal, scoring after the siren for the bonus point victory.
They sit on top of Pool C, with a bonus point victory from the Australians leapfrogging Wales and boosting their knockout hopes.